Bio.Restriction package
Submodules
Module contents
Restriction Digest Enzymes.
Examples
>>> from Bio.Seq import Seq >>> from Bio.Restriction import * >>> pBs_mcs = 'GGTACCGGGCCCCCCCTCGAGGTCGACGGTATCGATAAGCTTGATATCGAATTCCTG' >>> pBs_mcs += 'CAGCCCGGGGGATCCACTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCTC' >>> seq = Seq(pBs_mcs) # Multiple-cloning site of pBluescript SK(-) >>> a = Analysis(AllEnzymes, seq) >>> a.print_that() # no argument -> print all the results AbaSI : 10, 12, 13, 16, 17, 18, 19, 20, 22, 23, 24, 25, 25, 26, 27... BmeDI : 7, 8, 8, 9, 9, 13, 14, 15, 16, 17, 18, 19, 19, 21, 21... YkrI : 10, 12, 13, 16, 16, 17, 19, 20, 21, 22, 23, 24, 25, 25, 26...BmeDI : 1, 2, 7, 8, 8, 9, 9, 13, 14, 15, 16, 17, 18, 19… AccII : 98. AciI : 86, 90, 96, 98…
Enzymes which do not cut the sequence.
AspLEI BstHHI CfoI CviAII FaeI FaiI FatI GlaI HhaI Hin1II Hin6I HinP1I HpyCH4IV HpySE526I Hsp92II HspAI MaeII MseI NlaIII SaqAI TaiI Tru1I Tru9I… <BLANKLINE> >>> b = a.blunt() # Analysis with blunt enzmyes >>> a.print_that(b) # Print results for blunt cutters AccII : 98. AfaI : 4. AluBI : 40, 106. AluI : 40, 106. Bsh1236I : 98. BshFI : 10, 89. BsnI : 10, 89. BspANI : 10, 89…
Enzymes which do not cut the sequence.
FaiI GlaI CdiI MlyI SchI SspD5I AanI… <BLANKLINE>