Bio.Restriction package

Submodules

Module contents

Restriction Digest Enzymes.

Examples

>>> from Bio.Seq import Seq
>>> from Bio.Restriction import *
>>> pBs_mcs = 'GGTACCGGGCCCCCCCTCGAGGTCGACGGTATCGATAAGCTTGATATCGAATTCCTG'
>>> pBs_mcs += 'CAGCCCGGGGGATCCACTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCTC'
>>> seq = Seq(pBs_mcs)  # Multiple-cloning site of pBluescript SK(-)
>>> a = Analysis(AllEnzymes, seq)
>>> a.print_that()              # no argument -> print all the results
AbaSI      :  10, 12, 13, 16, 17, 18, 19, 20, 22, 23, 24, 25, 25, 26, 27...
BmeDI      :  7, 8, 8, 9, 9, 13, 14, 15, 16, 17, 18, 19, 19, 21, 21...
YkrI       :  10, 12, 13, 16, 16, 17, 19, 20, 21, 22, 23, 24, 25, 25, 26...
BmeDI : 1, 2, 7, 8, 8, 9, 9, 13, 14, 15, 16, 17, 18, 19… AccII : 98. AciI : 86, 90, 96, 98…

Enzymes which do not cut the sequence.

AspLEI BstHHI CfoI CviAII FaeI FaiI FatI GlaI HhaI Hin1II Hin6I HinP1I HpyCH4IV HpySE526I Hsp92II HspAI MaeII MseI NlaIII SaqAI TaiI Tru1I Tru9I… <BLANKLINE> >>> b = a.blunt() # Analysis with blunt enzmyes >>> a.print_that(b) # Print results for blunt cutters AccII : 98. AfaI : 4. AluBI : 40, 106. AluI : 40, 106. Bsh1236I : 98. BshFI : 10, 89. BsnI : 10, 89. BspANI : 10, 89…

Enzymes which do not cut the sequence.

FaiI GlaI CdiI MlyI SchI SspD5I AanI… <BLANKLINE>