- If you want help on using Bio.SeqIO, please join the discussion mailing list (see mailing lists).
- If you think you've found a bug, please report it on bugzilla.
- If you have a nice example you'd like to add, I would encourage you to join the discussion mailing list, but posting the code here should be fine.
New example, Random Fragments
I added a example into the output section, but now I wonder if this is the best place to put it. Maybe it should be under "examples". --Sbassi 01:20, 27 August 2007 (EDT)
Peter Nice idea - and yes, I would have put it under examples too. I've moved it and edited it too - I wanted the style to match the rest of the page, and also I think there was a possible problem in your randomising code where a sequence might run over the end of the record. I opted to simplify the example - I hope your still happy with it.
Hi, I am a newbie of Biopython. I tested Bio.SeqIO.parse on EMBL formated miRNA.dat from the microRNA Registry. But I got an error. Is the EMBL format supported fully? As:
>>>from Bio import SeqIO >>>handle = open('/home/liang/Desktop/miRNA.dat','rU') >>>record_iterator = SeqIO.parse(handle,'embl') >>>first_record=record_iterator.next() Traceback (most recent call last): File "<stdin>", line 1, in <module> File "/usr/lib/python2.5/site-packages/Bio/GenBank/Scanner.py", line 410, in parse_records record = self.parse(handle) File "/usr/lib/python2.5/site-packages/Bio/GenBank/Scanner.py", line 393, in parse if self.feed(handle, consumer) : File "/usr/lib/python2.5/site-packages/Bio/GenBank/Scanner.py", line 360, in feed self._feed_first_line(consumer, self.line) File "/usr/lib/python2.5/site-packages/Bio/GenBank/Scanner.py", line 540, in _feed_first_line assert len(fields) == 7 AssertionError
- Questions like this will probably be answered faster if you post them to the BioPython mail list. --Cjfields 16:03, 23 May 2007 (EDT)
- Peter as Chris suggested, your question would have been noticed much earlier on the mailing list - but filing a bug would have been an better idea (see bugzilla). Could you do that with a bit more information on where the example file came from? Thanks.
How to specify SeqRecord Alphabet
Hi, nice work with this module!
The to_dict() method creates a dictionary with biopython SeqRecord objects.
record_dict = SeqIO.to_dict(SeqIO.parse(handle, "fasta"))
How can I specify which Alphabet do they must have? By default, they have SingleLetterAlphabet.
Chromosome cneo_JEC21:cn-jec21_chr11: ID: cneo_JEC21:cn-jec21_chr11 Name: cneo_JEC21:cn-jec21_chr11 Desription: cneo_JEC21:cn-jec21_chr11 Seq('CCCCCTAACCCCCTAACCCCCTAACCCCCTAACCCCCTAACCCCTAACCCCCTAACCCTA ...', SingleLetterAlphabet())
Peter: The to_dict() method doesn't do anything with alphabets - your question is really about the parse() function. I've been thinking about extending parse() to take an optional alphabet to cover this exact situation where the file format doesn't specify the alphabet, but for now it doesn't. Sorry. If you are using FASTA files, and you need to specify the alphabet, then the best I can suggest is something like this:
Bio.Alphabet import generic_dna records = list(SeqIO.parse(handle, "fasta")) for record in records : record.seq.alphabet = generic_dna record_dict = SeqIO.to_dict(records)
Bio.Alphabet import generic_dna def force_dna(record) : record.seq.alphabet = generic_dna return record record_dict = SeqIO.to_dict(map(force_dna, SeqIO.parse(handle, "fasta")))
Or, if you like generators and are using Python 2.4 or later:
Bio.Alphabet import generic_dna def force_dna(record) : record.seq.alphabet = generic_dna return record record_dict = SeqIO.to_dict(force_dna(rec) for rec in SeqIO.parse(handle, "fasta")))
Why not join the discussion mailing list (see mailing lists) and tell us what you think?