Extract intergenic regions from a sequence. Sometimes, reading between the genes is more interesting....
The following ready-to-run script reads a genbank file, which is probably a genomic or chromosomal one. It uses the CDS feature to discover the 5' and 3' ends of ORFs. Yes, ORFs are not exactly synonymous with genes, but this is the way we did it. Also, you may want to swap the CDS feature for the 'gene' feature, if you are also interested in RNA coding genes. The "intergene_length" variable is a threshold on the minimal length of intergenic regions to be analyzed, and is set by default to 100. The program outputs to a file with the suffix ".ign" The program outputs the + strand or the reverse-complement based on the genbank file annotation. The output is in FASTA format, and the header includes the intergenic region coordinates, and unique ID, and whether the sequence was derived from the + or - strand.
#!/usr/bin/env python import sys import Bio from Bio import SeqIO, SeqFeature from Bio.SeqRecord import SeqRecord import os # Copyright(C) 2009 Iddo Friedberg & Ian MC Fleming # Released under Biopython license. http://www.biopython.org/DIST/LICENSE # Do not remove this comment def get_interregions(genbank_path,intergene_length=100): seq_record = SeqIO.read(open(genbank_path), "genbank") cds_list =  intergenic_records =  # Loop over the genome file, get the for fnum, feature in enumerate(seq_record.features): if feature.type == 'CDS': mystart = feature.location._start.position myend = feature.location._end.position cds_list.append((mystart,myend,feature.strand)) for i,pospair in enumerate(cds_list[1:]): # Compare current start position to previous end position last_end = cds_list[i] this_start = pospair strand = pospair if this_start - last_end >= intergene_length: intergene_seq = seq_record.seq[last_end:this_start] if strand == -1: intergene_seq = intergene_seq.reverse_complement() strand_string = "-" else: strand_string = "+" intergenic_records.append( SeqRecord(intergene_seq,id="%s-ign-%d" % (seq_record.name,i), description="%s %d-%d %s" % (seq_record.name, last_end+1, this_start,strand_string))) outpath = os.path.splitext(os.path.basename(genbank_path)) + ".ign" SeqIO.write(intergenic_records, open(outpath,"w"), "fasta") if __name__ == '__main__': if len(sys.argv) == 2: get_interregions(sys.argv) elif len(sys.argv) == 3: get_interregions(sys.argv,int(sys.argv)) else: print "Usage: get_intergenic.py gb_file [intergenic_length]" sys.exit(0)
./get_intergene mygenbankfile.gb 1
Run on the genbank file. The intergenic sequences will appear in the file mygenbank.ign in FASTA format.