Package Bio :: Package Restriction :: Module Restriction :: Class Blunt
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Type Blunt

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 object --+            
          |            
       type --+        
              |        
RestrictionType --+    
                  |    
        AbstractCut --+
                      |
                     Blunt
Known Subclasses:

Implement methods for enzymes that produce blunt ends.

The enzyme cuts the + strand and the - strand of the DNA at the same place.

Internal use only. Not meant to be instantiated.

Instance Methods [hide private]

Inherited from RestrictionType: __add__, __div__, __eq__, __floordiv__, __ge__, __gt__, __hash__, __init__, __le__, __len__, __lt__, __mod__, __ne__, __rdiv__, __repr__, __rfloordiv__, __rshift__, __rtruediv__, __str__, __truediv__

Inherited from type: __call__, __delattr__, __getattribute__, __instancecheck__, __new__, __setattr__, __subclasscheck__, __subclasses__, mro

Inherited from object: __format__, __reduce__, __reduce_ex__, __sizeof__, __subclasshook__

Class Methods [hide private]
 
catalyse(cls, dna, linear=True)
List the sequence fragments after cutting dna with enzyme.
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catalyze(cls, dna, linear=True)
List the sequence fragments after cutting dna with enzyme.
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is_blunt(cls)
Return if the enzyme produces blunt ends.
source code
 
is_5overhang(cls)
Return if the enzymes produces 5' overhanging ends.
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is_3overhang(cls)
Return if the enzyme produces 3' overhanging ends.
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overhang(cls)
Return the type of the enzyme's overhang as string.
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compatible_end(cls, batch=None)
List all enzymes that produce compatible ends for the enzyme.
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Inherited from AbstractCut: all_suppliers, equischizomers, frequency, is_equischizomer, is_isoschizomer, is_neoschizomer, isoschizomers, neoschizomers, search

Static Methods [hide private]
 
_mod1(other)
Test if other enzyme produces compatible ends for enzyme (PRIVATE).
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Properties [hide private]

Inherited from type: __abstractmethods__, __base__, __bases__, __basicsize__, __dictoffset__, __flags__, __itemsize__, __mro__, __name__, __weakrefoffset__

Inherited from object: __class__

Method Details [hide private]

catalyse(cls, dna, linear=True)
Class Method

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List the sequence fragments after cutting dna with enzyme.

RE.catalyze(dna, linear=True) -> tuple of DNA.

Return a tuple of dna as will be produced by using RE to restrict the dna.

dna must be a Bio.Seq.Seq instance or a Bio.Seq.MutableSeq instance.

If linear is False, the sequence is considered to be circular and the output will be modified accordingly.

catalyze(cls, dna, linear=True)
Class Method

source code 

List the sequence fragments after cutting dna with enzyme.

RE.catalyze(dna, linear=True) -> tuple of DNA.

Return a tuple of dna as will be produced by using RE to restrict the dna.

dna must be a Bio.Seq.Seq instance or a Bio.Seq.MutableSeq instance.

If linear is False, the sequence is considered to be circular and the output will be modified accordingly.

is_blunt(cls)
Class Method

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Return if the enzyme produces blunt ends.

True if the enzyme produces blunt end.

Related methods:
  • RE.is_3overhang()
  • RE.is_5overhang()
  • RE.is_unknown()

is_5overhang(cls)
Class Method

source code 

Return if the enzymes produces 5' overhanging ends.

True if the enzyme produces 5' overhang sticky end.

Related methods:
  • RE.is_3overhang()
  • RE.is_blunt()
  • RE.is_unknown()

is_3overhang(cls)
Class Method

source code 

Return if the enzyme produces 3' overhanging ends.

True if the enzyme produces 3' overhang sticky end.

Related methods:
  • RE.is_5overhang()
  • RE.is_blunt()
  • RE.is_unknown()

overhang(cls)
Class Method

source code 

Return the type of the enzyme's overhang as string.

Can be "3' overhang", "5' overhang", "blunt", "unknown".

_mod1(other)
Static Method

source code 

Test if other enzyme produces compatible ends for enzyme (PRIVATE).

For internal use only

Test for the compatibility of restriction ending of RE and other.